human SUGT1 shRNA silencing AAV

Name
AAV-h-SUGT1-shRNA

Cat No
shAAV-224701

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human SUGT1.

shAAV-224701

AAV-h-SUGT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SUGT1 (SGT1 homolog, MIS12 kinetochore complex assembly cochaperone). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Protein 40-6-3; protein SGT1 homolog; putative 40-6-3 protein; SGT1; SGT1, suppressor of G2 allele of SKP1; Suppressor of G2 allele of SKP1 homolog; suppressor of G2 allele of SKP1, S. cerevisiae, homolog of

Description (eCommerce)

Sgt1 (suppressor of G2 allelle of SKP1 homolog) is a component of the Hsp90-Sgt1 chaperone complex which has been shown to play a role in kinetochore assembly. Sgt1 is also reported to be a component of the SCF ubiquitin ligase and may play a role in ubiquitination.

Description (Vector)

SUGT1 is homologous to the yeast gene SGT1, which encodes a protein involved in kinetochore function and required for the G1/S and G2/M transitions. Complementation studies suggest that the human protein has similar functions. Two transcript variants encoding different isoforms have been found for this gene.

Gene ID

10910

Gene Name (eCommerce)

SGT1 homolog, MIS12 kinetochore complex assembly cochaperone

Gene Name (Vector)

SGT1 homolog, MIS12 kinetochore complex assembly cochaperone

Gene Symbol

SUGT1

HGNC ID

HGNC:16987

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1002

Protein Name (eCommerce)

Protein SGT1 homolog

RefSeq ID

NM_006704

RefSeq Synonyms

NM_006704, NM_001320831, NM_001130912, BC000911,

Research Areas

Cell Cycle,Immunology,Innate Immunity,Ubiquitination

Research Areas (Faceted)

cell_biology,cell_cycle,immunology,genetics

Species

human

Target Sentence

Sgt1 (suppressor of G2 allelle of SKP1 homolog) is a component of the Hsp90-Sgt1 chaperone complex which has been shown to play a role in kinetochore assembly. Sgt1 is also reported to be a component of the SCF ubiquitin ligase and may play a role in ubiquitination.

UniGene ID

Hs.281902

UniProt ID (eCommerce)

Q9Y2Z0

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.