human SPTLC3 shRNA silencing AAV

Name
AAV-h-SPTLC3-shRNA

Cat No
shAAV-224296

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human SPTLC3.

shAAV-224296

AAV-h-SPTLC3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SPTLC3 (serine palmitoyltransferase long chain base subunit 3). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB2B; LCB3; SPT3; SPTLC2L

Description (Vector)

The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50), which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al., 2006 (PubMed 17023427)). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3), depending on the tissue in which biosynthesis occurs (Hornemann et al., 2006 (PubMed 17023427)). There are also 2 highly related isoforms of a third subunit, SSSPTA (MIM 613540) and SSSPTB (MIM 610412), that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al., 2009 (PubMed 19416851)).(supplied by OMIM, Nov 2010)

Gene ID

55304

Gene Name (Vector)

serine palmitoyltransferase long chain base subunit 3

Gene Symbol

SPTLC3

HGNC ID

HGNC:16253

ORF Size (aa)

1659

ORF Size (bp)

1659 bp

RefSeq ID

NM_018327

RefSeq Synonyms

NM_018327, BC150644, BC020656,

Species

human

UniGene ID

Hs.425023

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.