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human SPRED1 shRNA silencing AAV

Name 
AAV-h-SPRED1-shRNA
Cat No 
shAAV-224240
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SPRED1.

shAAV-224240
AAV-h-SPRED1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SPRED1 (sprouty related EVH1 domain containing 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hSpred1; NFLS; PPP1R147; spred-1
Description (Vector)
SPRED1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
Gene ID
161742
Gene Name (Vector)
sprouty related EVH1 domain containing 1
Gene Symbol
SPRED1
HGNC ID
HGNC:20249
ORF Size (aa)
1335
ORF Size (bp)
1335 bp
RefSeq ID
NM_152594
RefSeq Synonyms
NM_152594, BC137481, BC137480,
Species
human
UniGene ID
Hs.525781

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.