human SPI1 shRNA silencing AAV

Name 
AAV-h-SPI1-shRNA
Cat No 
shAAV-224172
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SPI1.

shAAV-224172
AAV-h-SPI1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SPI1 (Spi-1 proto-oncogene). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1; transcription factor PU.1
Description (eCommerce)
PU.1 is an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. PU.1 binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. [taken from NCBI Entrez Gene (Gene ID: 6688)].
Description (Vector)
SPI1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
6688
Gene Name (eCommerce)
Spi-1 proto-oncogene
Gene Name (Vector)
Spi-1 proto-oncogene
Gene Symbol
SPI1
HGNC ID
HGNC:11241
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
792
Protein Name (eCommerce)
Transcription factor PU.1
RefSeq ID
NM_003120
RefSeq Synonyms
NM_003120, NM_001080547, BC111379,
Research Areas
Apoptosis,Cancer,Epigenetic Regulation of Gene Expression,FGF Signaling,Gene Expression,Histone Modification,Host-Virus Interactions,IFN Signaling,Immunology,Interleukin Signaling,Leukemia,RANKL-RANK Signaling,RNA Binding,Signal Transduction,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer
Research Areas (Faceted)
cancer,cell_biology,genetics,immunology,infectious_disease,signal_transduction,transcription_translation
Species
human
Target Sentence
PU.1 is an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. PU.1 binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. [taken from NCBI Entrez Gene (Gene ID: 6688)].
UniGene ID
Hs.502511
UniProt ID (eCommerce)
P17947

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.