human SPAG5 shRNA silencing AAV

Name 
AAV-h-SPAG5-shRNA
Cat No 
shAAV-224068
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SPAG5.

shAAV-224068
AAV-h-SPAG5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SPAG5 (sperm associated antigen 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
astrin; DEEPEST; hMAP126; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; Mitotic spindle-associated protein p126; sperm-associated antigen 5
Description (eCommerce)
Astrin/MAP126 has been identified as a mitotic spindle-associated protein essential for centrosome integrity and the progression through mitosis. Astrin/MAP126 interacts with Ninein and Aurora-A at the centrosome. Ninein, but not Aurora-A, appears to be required for proper localization of Astrin/MAP126 to the centrosome during S and G2 phase. A pool of Astrin/MAP126 also localizes to the outer kinetochore where it plays an essential role in chromatid adhesion and chromosome congression and segregation. The localization of Astrin/MAP126 to the spindle pole and kinetochore has been shown to be dependent on its phosphorylation by glycogen synthase kinase 3beta (GSK3beta).
Description (Vector)
SPAG5 encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.
Gene ID
10615
Gene Name (eCommerce)
sperm associated antigen 5
Gene Name (Vector)
sperm associated antigen 5
Gene Symbol
SPAG5
HGNC ID
HGNC:13452
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3582
ORF Size (bp)
3582 bp
Protein Name (eCommerce)
Sperm-associated antigen 5
RefSeq ID
NM_006461
RefSeq Synonyms
NM_006461, BC000322,
Research Areas
Cell Cycle,Cell Division,Cytoskeleton,Signal Transduction
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,signal_transduction
Species
human
Target Sentence
Astrin/MAP126 has been identified as a mitotic spindle-associated protein essential for centrosome integrity and the progression through mitosis. Astrin/MAP126 interacts with Ninein and Aurora-A at the centrosome. Ninein, but not Aurora-A, appears to be required for proper localization of Astrin/MAP126 to the centrosome during S and G2 phase. A pool of Astrin/MAP126 also localizes to the outer kinetochore where it plays an essential role in chromatid adhesion and chromosome congression and segregation. The localization of Astrin/MAP126 to the spindle pole and kinetochore has been shown to be dependent on its phosphorylation by glycogen synthase kinase 3beta (GSK3beta).
UniGene ID
Hs.514033
UniProt ID (eCommerce)
Q96R06

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.