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human SMARCD1 shRNA silencing AAV

Name 
AAV-h-SMARCD1-shRNA
Cat No 
shAAV-223724
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SMARCD1.

shAAV-223724
AAV-h-SMARCD1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
60 kDa BRG-1/Brm-associated factor subunit A; BAF60A; BRG1-associated factor 60A; chromatin remodeling complex BAF60A subunit; CRACD1; mammalian chromatin remodeling complex BRG1-associated factor 60A; Rsc6p; SWI/SNF complex 60 kDa subunit; SWI/SNF complex 60 kDa subunit A; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; Swp73-like protein
Description (eCommerce)
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCD1/BAF60a is subunit of the SWI/SNF complex that has been identified as a factor that interacts with nuclear receptors such as the vitamin D receptor and glucocorticoid receptor and a subset of Fos/Jun dimers. SMARCD1 has also been shown to interact with p53 and is suggested to be involved in the tumor suppressor activity of p53. From these findings it has been concluded that SMARCD1 functions as a direct link between transcription factors and SWI/SNF remodeling activity to regulate transcription.
Description (Vector)
SMARCD1 is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
6602
Gene Name (eCommerce)
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Gene Name (Vector)
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Gene Symbol
SMARCD1
HGNC ID
HGNC:11106
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1431
ORF Size (bp)
1431 bp
Protein Name (eCommerce)
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1
RefSeq ID
NM_003076
RefSeq Synonyms
NM_139071, NM_003076, BC009368,
Research Areas
Cancer,Chromatin Remodeling,Neurobiology,Neurodevelopment,Neurogenesis,Prostate Cancer,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,transcription_translation,neurobiology,developmental_biology,genetics,transcription_translation
Species
human
Target Sentence
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCD1/BAF60a is subunit of the SWI/SNF complex that has been identified as a factor that interacts with nuclear receptors such as the vitamin D receptor and glucocorticoid receptor and a subset of Fos/Jun dimers. SMARCD1 has also been shown to interact with p53 and is suggested to be involved in the tumor suppressor activity of p53. From these findings it has been concluded that SMARCD1 functions as a direct link between transcription factors and SWI/SNF remodeling activity to regulate transcription.
UniGene ID
Hs.79335
UniProt ID (eCommerce)
Q96GM5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.