human SAE1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human SAE1.
shAAV-222197
AAV-h-SAE1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human SAE1 (SUMO1 activating enzyme subunit 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
activator of SUMO1; AOS1; HSPC140; sentrin/SUMO-activating protein AOS1; SUA1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A
Description (eCommerce)
Heterodimers of SUMO-activating enzyme subunit 1 (SAE1) and SAE2 act as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. Heterodimers mediate ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UBE0].
Description (Vector)
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 (PubMed 9920803)).(supplied by OMIM, Mar 2010)
Gene ID
10055
Gene Name (eCommerce)
SUMO1 activating enzyme subunit 1
Gene Name (Vector)
SUMO1 activating enzyme subunit 1
Gene Symbol
SAE1
HGNC ID
HGNC:30660
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1041
ORF Size (bp)
1041 bp
Protein Name (eCommerce)
SUMO-activating enzyme subunit 1
RefSeq ID
NM_005500
RefSeq Synonyms
NM_005500, NM_001145714, NM_001145713, BC018271, BC003611, BC000344,
Research Areas
Cell Cycle,Ubiquitination
Research Areas (Faceted)
cell_biology,cell_cycle,genetics
Species
human
Target Sentence
Heterodimers of SUMO-activating enzyme subunit 1 (SAE1) and SAE2 act as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. Heterodimers mediate ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UBE0].
UniGene ID
Hs.515500
UniProt ID (eCommerce)
Q9UBE0
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.