human RPL35A shRNA silencing AAV

Name 
AAV-h-RPL35A-shRNA
Cat No 
shAAV-221685
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RPL35A.

shAAV-221685
AAV-h-RPL35A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RPL35A (ribosomal protein L35a). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
60S ribosomal protein L35a; cell growth-inhibiting gene 33 protein; DBA5; eL33; L35A; large ribosomal subunit protein eL33
Description (eCommerce)
The RPL35A gene encodes for 60S ribosomal protein L35a. The protein belongs to the L35AE family of ribosomal proteins. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome [taken from NCBI Entrez Gene (Gene ID: 6165)].
Description (Vector)
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Transcript variants utilizing alternative transcription initiation sites and alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Gene ID
6165
Gene Name (eCommerce)
ribosomal protein L35a
Gene Name (Vector)
ribosomal protein L35a
Gene Symbol
RPL35A
HGNC ID
HGNC:10345
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
333
Protein Name (eCommerce)
60S ribosomal protein L35a
RefSeq ID
NM_000996
RefSeq Synonyms
NM_001316311, NM_000996, BC061890, BC017093, BC010949, BC001037,
Research Areas
Gene Expression,Host-Virus Interactions,Metabolism/Metabolic Process,RNA Binding,RNA Processing,Translational Control,tRNA Binding
Research Areas (Faceted)
cell_biology,genetics,infectious_disease,metabolism,transcription_translation
Species
human
Target Sentence
The RPL35A gene encodes for 60S ribosomal protein L35a. The protein belongs to the L35AE family of ribosomal proteins. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome [taken from NCBI Entrez Gene (Gene ID: 6165)].
UniGene ID
Hs.529631
UniProt ID (eCommerce)
P18077

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.