human ROCK1 shRNA silencing AAV

Name 
AAV-h-ROCK1-shRNA
Cat No 
shAAV-221475
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ROCK1.

shAAV-221475
AAV-h-ROCK1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ROCK1 (Rho associated coiled-coil containing protein kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
p160 ROCK-1; P160ROCK; renal carcinoma antigen NY-REN-35; rho-associated protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I
Description (eCommerce)
Rho-associated protein kinase 1 (ROCK1), also known as Rho kinase beta, is a serine-threonine protein kinase that plays an important role in cellular apoptosis, growth, cell motility and cell contraction. ROCK1 shares an overall 65% homology in amino acid sequence with ROCK2. The ROCK1 and ROCK2 isoforms are products of two separate genes. Both ROCK1 and ROCK2 are downstream targets of the Rho A small GTP-binding protein. As downstream effectors of RhoA, ROCK1 and ROCK2 mediate changes in the actin cytoskeleton via phosphorylation of target proteins such as the MBS subunit of MLCP, MLC, ERM proteins, protein LIM kinase and adducin.
Description (Vector)
ROCK1 encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Gene ID
6093
Gene Name (eCommerce)
Rho associated coiled-coil containing protein kinase 1
Gene Name (Vector)
Rho associated coiled-coil containing protein kinase 1
Gene Symbol
ROCK1
HGNC ID
HGNC:10251
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4065
ORF Size (bp)
4065 bp
Protein Name (eCommerce)
Rho-associated protein kinase 1
RefSeq ID
NM_005406
RefSeq Synonyms
NM_005406, BC113114,
Research Areas
Angiogenesis,Apoptosis,Axon Guidance,Cancer,Cardiac Hypertrophy,Cardiology,Cardiovascular,Cytoskeleton,Golgi Apparatus,GPCR Signaling,Immunology,Kinase/Phosphatase,MicroRNAs in Cancer,Neurobiology,Neurodevelopment,Rho Signaling,Signal Transduction,TGF-beta Signaling,VEGF Signaling,Wnt Signaling,Zinc-finger
Research Areas (Faceted)
cancer,genetics,signal_transduction,developmental_biology,cell_biology,neurobiology,cardiovascular,cell_biology,signal_transduction,immunology,cell_biology,neurobiology,developmental_biology,signal_transduction,immunology,developmental_biology,cell_biology
Species
human
Target Sentence
Rho-associated protein kinase 1 (ROCK1), also known as Rho kinase beta, is a serine-threonine protein kinase that plays an important role in cellular apoptosis, growth, cell motility and cell contraction. ROCK1 shares an overall 65% homology in amino acid sequence with ROCK2. The ROCK1 and ROCK2 isoforms are products of two separate genes. Both ROCK1 and ROCK2 are downstream targets of the Rho A small GTP-binding protein. As downstream effectors of RhoA, ROCK1 and ROCK2 mediate changes in the actin cytoskeleton via phosphorylation of target proteins such as the MBS subunit of MLCP, MLC, ERM proteins, protein LIM kinase and adducin.
UniGene ID
Hs.306307
UniProt ID (eCommerce)
Q13464

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.