human RFC5 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human RFC5.
shAAV-221041
AAV-h-RFC5-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human RFC5 (replication factor C subunit 5). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
A1 36 kDa subunit; Activator 1 36 kDa subunit; Activator 1 subunit 5; replication factor C (activator 1) 5, 36.5kDa; Replication factor C 36 kDa subunit; replication factor C subunit 5; replication factor C, 36-kDa subunit; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; RFC36
Description (eCommerce)
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC5 is the 36kDa subunit of RFC that forms a core complex with RFC2 and RFC4 and can interact with the C-terminal region of PCNA.
Description (Vector)
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.
Gene ID
5985
Gene Name (eCommerce)
replication factor C subunit 5
Gene Name (Vector)
replication factor C subunit 5
Gene Symbol
RFC5
HGNC ID
HGNC:9973
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1023
Protein Name (eCommerce)
Replication factor C subunit 5
RefSeq ID
NM_007370
RefSeq Synonyms
NM_181578, NM_007370, NM_001346815, NM_001206801, NM_001130113, NM_001130112, BC013961, BC001866,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Homologous Recombination,Replication,Signal Transduction,Telomeric Maintenance
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC5 is the 36kDa subunit of RFC that forms a core complex with RFC2 and RFC4 and can interact with the C-terminal region of PCNA.
UniGene ID
Hs.506989
UniProt ID (eCommerce)
P40937
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.