human RFC1 shRNA silencing AAV

Name 
AAV-h-RFC1-shRNA
Cat No 
shAAV-221036
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RFC1.

shAAV-221036
AAV-h-RFC1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RFC1 (replication factor C subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A1; A1 140 kDa subunit; activator 1 140 kDa subunit; activator 1 large subunit; activator 1 subunit 1; DNA-binding protein PO-GA; MHC binding factor, beta; MHCBFB; PO-GA; RECC1; replication factor C (activator 1) 1, 145kDa; replication factor C 140 kDa subunit; replication factor C large subunit; replication factor C subunit 1; replication factor C1; RFC; RF-C 140 kDa subunit; RFC140
Description (eCommerce)
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC1 is the 140 kDa subunit of RFC that has a DNA-binding domain with a BRCT motif and is suggested to play a more critical role than the other subunits.
Description (Vector)
RFC1 encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Gene ID
5981
Gene Name (eCommerce)
replication factor C subunit 1
Gene Name (Vector)
replication factor C subunit 1
Gene Symbol
RFC1
HGNC ID
HGNC:9969
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3444
ORF Size (bp)
3444 bp
Protein Name (eCommerce)
Replication factor C subunit 1
RefSeq ID
NM_002913
RefSeq Synonyms
NM_002913, NM_001204747, BC051786, BC051751, BC035297,
Research Areas
Cardiology,Cardiovascular,CD95/FAS Signaling,Cell Cycle,DNA Damage/Repair,DNA Replication,Homologous Recombination,Signal Transduction,Telomeric Maintenance,Transcription Factor/Regulator
Research Areas (Faceted)
signal_transduction,cardiovascular,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC1 is the 140 kDa subunit of RFC that has a DNA-binding domain with a BRCT motif and is suggested to play a more critical role than the other subunits.
UniGene ID
Hs.507475
UniProt ID (eCommerce)
P35251

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.