human RAD50 shRNA silencing AAV

Name 
AAV-h-RAD50-shRNA
Cat No 
shAAV-220642
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RAD50.

shAAV-220642
AAV-h-RAD50-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RAD50 (RAD50 double strand break repair protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DNA repair protein RAD50; hRad50; NBSLD; RAD50 homolog, double strand break repair protein; RAD502
Description (eCommerce)
Rad50 is a component of the Mre11-Rad50-Nbs1 (MRN) complex that repairs DNA double strand breaks during DNA damage and homologous recombination and activates the S-phase checkpoint. Rad50 shows structural similarity to the structural maintenance of chromosomes (SMC) family of proteins. Rad50 has been shown to directly interact with BRCA2, TERF2IP, and ATM.
Description (Vector)
RAD50 is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Gene ID
10111
Gene Name (eCommerce)
RAD50 double strand break repair protein
Gene Name (Vector)
RAD50 double strand break repair protein
Gene Symbol
RAD50
HGNC ID
HGNC:9816
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3939
ORF Size (bp)
3939 bp
Protein Name (eCommerce)
DNA repair protein RAD50
RefSeq ID
NM_005732
RefSeq Synonyms
NM_005732, BC136436,
Research Areas
Breast Cancer,Cancer,Cell Cycle,DNA Damage/Repair,Fanconi anemia,Helicase Activity,Homologous Recombination,Meiosis,Prostate Cancer,Signal Transduction,Telomeric Maintenance
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
Rad50 is a component of the Mre11-Rad50-Nbs1 (MRN) complex that repairs DNA double strand breaks during DNA damage and homologous recombination and activates the S-phase checkpoint. Rad50 shows structural similarity to the structural maintenance of chromosomes (SMC) family of proteins. Rad50 has been shown to directly interact with BRCA2, TERF2IP, and ATM.
UniGene ID
Hs.655835
UniProt ID (eCommerce)
Q92878

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.