human PTGIS shRNA silencing AAV

Name
AAV-h-PTGIS-shRNA

Cat No
shAAV-220252

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PTGIS.

shAAV-220252

AAV-h-PTGIS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PTGIS (prostaglandin I2 synthase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CYP8; CYP8A1; PGIS; PTGI

Description (Vector)

PTGIS encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis.

Gene ID

5740

Gene Name (Vector)

prostaglandin I2 synthase

Gene Symbol

PTGIS

HGNC ID

HGNC:9603

ORF Size (aa)

1503

ORF Size (bp)

1503 bp

RefSeq ID

NM_000961

RefSeq Synonyms

NM_000961, BC101811, BC101809,

Species

human

UniGene ID

Hs.302085

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.