human PSMF1 shRNA silencing AAV

Name 
AAV-h-PSMF1-shRNA
Cat No 
shAAV-220185
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PSMF1.

shAAV-220185
AAV-h-PSMF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PSMF1 (proteasome inhibitor subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hPI31; PI31; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); proteasome inhibitor PI31 subunit
Description (eCommerce)
PSMF1 [proteasome (prosome, macropain) inhibitor subunit 1 (PI31)] plays an important role in control of proteasome function. It inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. PSMF1 also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92530].
Description (Vector)
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
Gene ID
9491
Gene Name (eCommerce)
proteasome inhibitor subunit 1
Gene Name (Vector)
proteasome inhibitor subunit 1
Gene Symbol
PSMF1
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
816
Protein Name (eCommerce)
Proteasome inhibitor PI31 subunit
RefSeq ID
NM_006814
RefSeq Synonyms
NM_178578, NM_006814, NM_001323410, NM_001323409, NM_001323408, NM_001323407, BC126462, BC029836,
Research Areas
Antigen Processing and Presentation,Apoptosis,Cell Cycle,Cell Division,Complexome,DNA Damage/Repair,Gene Expression,Host-Virus Interactions,Immunology,Metabolism/Metabolic Process,Proteasome,RNA Processing,Ubiquitination
Research Areas (Faceted)
immunology,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,metabolism
Species
human
Target Sentence
PSMF1 [proteasome (prosome, macropain) inhibitor subunit 1 (PI31)] plays an important role in control of proteasome function. It inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. PSMF1 also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92530].
UniGene ID
Hs.471917
UniProt ID (eCommerce)
Q92530

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.