human PRNP shRNA silencing AAV

Name
AAV-h-PRNP-shRNA

Cat No
shAAV-219911

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PRNP.

shAAV-219911

AAV-h-PRNP-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRNP (prion protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc

Description (Vector)

PRNP is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein.

Gene ID

5621

Gene Name (Vector)

prion protein

Gene Symbol

PRNP

HGNC ID

HGNC:9449

ORF Size (aa)

762

RefSeq ID

NM_000311

RefSeq Synonyms

NM_183079, NM_001271561, NM_001080123, NM_001080122, NM_001080121, NM_000311, BC022532, BC012844,

Species

human

UniGene ID

Hs.714553

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.