human PRKAG3 shRNA silencing AAV

Name
AAV-h-PRKAG3-shRNA

Cat No
shAAV-219847

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PRKAG3.

shAAV-219847

AAV-h-PRKAG3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRKAG3 (protein kinase AMP-activated non-catalytic subunit gamma 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AMPKG3

Description (Vector)

PRKAG3 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Gene ID

53632

Gene Name (Vector)

protein kinase AMP-activated non-catalytic subunit gamma 3

Gene Symbol

PRKAG3

HGNC ID

HGNC:9387

ORF Size (aa)

1470

ORF Size (bp)

1470 bp

RefSeq ID

NM_017431

RefSeq Synonyms

NM_017431, BC098306, BC098277, BC098255, BC098102,

Species

human

UniGene ID

Hs.591634

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.