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human PPM1F shRNA silencing AAV

Name 
AAV-h-PPM1F-shRNA
Cat No 
shAAV-219580
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PPM1F.

shAAV-219580
AAV-h-PPM1F-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPM1F (protein phosphatase, Mg2+/Mn2+ dependent 1F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2
Description (Vector)
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
Gene ID
9647
Gene Name (Vector)
protein phosphatase, Mg2+/Mn2+ dependent 1F
Gene Symbol
PPM1F
HGNC ID
HGNC:19388
ORF Size (aa)
1362
ORF Size (bp)
1362 bp
RefSeq ID
NM_014634
RefSeq Synonyms
NM_014634, BC105599, BC071989,
Species
human
UniGene ID
Hs.112728

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.