human PPEF1 shRNA silencing AAV

Name
AAV-h-PPEF1-shRNA

Cat No
shAAV-219522

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PPEF1.

shAAV-219522

AAV-h-PPEF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPEF1 (protein phosphatase with EF-hand domain 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PP7; PPEF; PPP7C; PPP7CA

Description (Vector)

PPEF1 encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. PPEF1 shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described.

Gene ID

5475

Gene Name (Vector)

protein phosphatase with EF-hand domain 1

Gene Symbol

PPEF1

HGNC ID

HGNC:9243

ORF Size (aa)

1962

ORF Size (bp)

1962 bp

RefSeq ID

NM_006240

RefSeq Synonyms

NM_152226, NM_152224, NM_006240, BC036026,

Species

human

UniGene ID

Hs.211589

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.