human PNPLA1 shRNA silencing AAV

Name
AAV-h-PNPLA1-shRNA

Cat No
shAAV-219311

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PNPLA1.

shAAV-219311

AAV-h-PNPLA1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PNPLA1 (patatin like phospholipase domain containing 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ARCI10; dJ50J22.1

Description (Vector)

PNPLA1 belongs to the patatin-like phospholipase (PNPLA) family, which is characterized by the presence of a highly conserved patatin domain. PNPLA family members have diverse lipolytic and acyltransferase activities, and are key elements in lipid metabolism. While other members of this family have been well characterized, the function of this gene remained an enigma. However, recent studies show that this gene is expressed in the skin epidermal keratinocytes, and has a role in glycerophospholipid metabolism in the cutaneous barrier. Consistent with these observations, mutations in this gene are associated with ichthyosis in human (autosomal recessive congenital ichthyoses, ARCI) and dog. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

285848

Gene Name (Vector)

patatin like phospholipase domain containing 1

Gene Symbol

PNPLA1

HGNC ID

HGNC:21246

ORF Size (aa)

1341

ORF Size (bp)

1341 bp

RefSeq ID

NM_173676

RefSeq Synonyms

NM_173676, NM_001145717, NM_001145716, BC103907, BC103906, BC103905,

Species

human

UniGene ID

Hs.407002

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.