human PLOD3 shRNA silencing AAV

Name
AAV-h-PLOD3-shRNA

Cat No
shAAV-219220

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PLOD3.

shAAV-219220

AAV-h-PLOD3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PLOD3 (procollagen-lysine,2-oxoglutarate 5-dioxygenase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

LH3

Description (Vector)

PLOD3 is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.

Gene ID

8985

Gene Name (Vector)

procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

Gene Symbol

PLOD3

HGNC ID

HGNC:9083

ORF Size (aa)

2217

ORF Size (bp)

2217 bp

RefSeq ID

NM_001084

RefSeq Synonyms

NM_001084, BC011674,

Species

human

UniGene ID

Hs.153357

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.