human PLOD2 shRNA silencing AAV

Name
AAV-h-PLOD2-shRNA

Cat No
shAAV-219219

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PLOD2.

shAAV-219219

AAV-h-PLOD2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PLOD2 (procollagen-lysine,2-oxoglutarate 5-dioxygenase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BRKS2; LH2; TLH

Description (Vector)

PLOD2 is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene ID

5352

Gene Name (Vector)

procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

Gene Symbol

PLOD2

HGNC ID

HGNC:9082

ORF Size (aa)

2277

ORF Size (bp)

2277 bp

RefSeq ID

NM_000935

RefSeq Synonyms

NM_182943, NM_000935, BC037169,

Species

human

UniGene ID

Hs.477866

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.