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human PIGT shRNA silencing AAV

Name 
AAV-h-PIGT-shRNA
Cat No 
shAAV-218923
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PIGT.

shAAV-218923
AAV-h-PIGT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PIGT (phosphatidylinositol glycan anchor biosynthesis class T). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CGI-06; MCAHS3; NDAP; PNH2
Description (Vector)
PIGT encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
51604
Gene Name (Vector)
phosphatidylinositol glycan anchor biosynthesis class T
Gene Symbol
PIGT
HGNC ID
HGNC:14938
ORF Size (aa)
1737
ORF Size (bp)
1737 bp
RefSeq ID
NM_015937
RefSeq Synonyms
NM_015937, NM_001184730, NM_001184729, NM_001184728, BC136828, BC136827, BC015022,
Species
human
UniGene ID
Hs.437388

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.