human PFKM shRNA silencing AAV

Name 
AAV-h-PFKM-shRNA
Cat No 
shAAV-218684
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PFKM.

shAAV-218684
AAV-h-PFKM-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PFKM (phosphofructokinase, muscle). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
6-phosphofructo-1-kinase; 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; GSD7; PFK1; PFK-1; PFKA; PFK-A; PFK-M; PFKX; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase, polypeptide X; phosphofructokinase-M; phosphohexokinase; PPP1R122; protein phosphatase 1, regulatory subunit 122
Description (eCommerce)
PFKM catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08237].
Description (Vector)
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
Gene ID
5213
Gene Name (eCommerce)
phosphofructokinase, muscle
Gene Name (Vector)
phosphofructokinase, muscle
Gene Symbol
PFKM
HGNC ID
HGNC:8877
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2343
ORF Size (bp)
2343 bp
Protein Name (eCommerce)
ATP-dependent 6-phosphofructokinase, muscle type
RefSeq ID
NM_000289
RefSeq Synonyms
NM_001166688, NM_001166687, NM_001166686, NM_000289, BC021203, BC013298, BC012799, BC000534,
Research Areas
Insulin Signaling,Kinase/Phosphatase,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
signal_transduction,metabolism,cell_biology
Species
human
Target Sentence
PFKM catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08237].
UniGene ID
Hs.75160
UniProt ID (eCommerce)
P08237

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.