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human PEMT shRNA silencing AAV

Name 
AAV-h-PEMT-shRNA
Cat No 
shAAV-218615
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PEMT.

shAAV-218615
AAV-h-PEMT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PEMT (phosphatidylethanolamine N-methyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PEAMT; PEMPT; PEMT2; PLMT; PNMT
Description (Vector)
Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Gene ID
10400
Gene Name (Vector)
phosphatidylethanolamine N-methyltransferase
Gene Symbol
PEMT
HGNC ID
HGNC:8830
ORF Size (aa)
711
RefSeq ID
NM_007169
RefSeq Synonyms
NM_148173, NM_148172, NM_007169, NM_001267552, NM_001267551, BC050593, BC000557,
Species
human
UniGene ID
Hs.287717

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.