human PDE8B shRNA silencing AAV

Name
AAV-h-PDE8B-shRNA

Cat No
shAAV-218498

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PDE8B.

shAAV-218498

AAV-h-PDE8B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PDE8B (phosphodiesterase 8B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

3',5' cyclic nucleotide phosphodiesterase 8B; ADSD; cell proliferation-inducing gene 22 protein; high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B; HsPDE8B; PPNAD3

Description (eCommerce)

Phosphodiesterase 8B is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. PDE8B, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in the PDE8B gene are a cause of autosomal dominant striatal degeneration (ADSD) [taken from NCBI Entrez Gene (Gene ID: 8622)].

Description (Vector)

PDE8B is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.

Gene ID

8622

Gene Name (eCommerce)

phosphodiesterase 8B

Gene Name (Vector)

phosphodiesterase 8B

Gene Symbol

PDE8B

HGNC ID

HGNC:8794

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2658

ORF Size (bp)

2658 bp

Protein Name (eCommerce)

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

RefSeq ID

NM_003719

RefSeq Synonyms

NM_003719, NM_001029854, NM_001029853, NM_001029852, NM_001029851, BC156987, BC156215,

Research Areas

GPCR Signaling,Metabolism/Metabolic Process,Neurobiology,Signal Transduction

Research Areas (Faceted)

cell_biology,metabolism,neurobiology,signal_transduction

Species

human

Target Sentence

Phosphodiesterase 8B is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. PDE8B, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in the PDE8B gene are a cause of autosomal dominant striatal degeneration (ADSD) [taken from NCBI Entrez Gene (Gene ID: 8622)].

UniGene ID

Hs.584830

UniProt ID (eCommerce)

O95263

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.