human PCNA shRNA silencing AAV

Name 
AAV-h-PCNA-shRNA
Cat No 
shAAV-218393
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PCNA.

shAAV-218393
AAV-h-PCNA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PCNA (proliferating cell nuclear antigen). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATLD2; cyclin; DNA polymerase delta auxiliary protein; PCNA; proliferating cell nuclear antigen
Description (eCommerce)
PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
Description (Vector)
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Gene ID
5111
Gene Name (eCommerce)
proliferating cell nuclear antigen
Gene Name (Vector)
proliferating cell nuclear antigen
Gene Symbol
PCNA
HGNC ID
HGNC:8729
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
786
Protein Name (eCommerce)
Proliferating cell nuclear antigen
RefSeq ID
NM_002592
RefSeq Synonyms
NM_182649, NM_002592, BC062439, BC000491,
Research Areas
Autophagy,Cancer,Cardiac Development,Cardiology,Cardiovascular,Cell Cycle,Cell Division,Cytoskeleton,DNA Damage/Repair,DNA Replication,EGFR Signaling,Host-Virus Interactions,Immunology,Interleukin Signaling,p53 Signaling,Phospho-specific,Replication,Signal Transduction,Telomeric Maintenance,Transport
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,signal_transduction,transcription_translation
Species
human
Target Sentence
PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
UniGene ID
Hs.147433
UniProt ID (eCommerce)
P12004

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.