human PCCB shRNA silencing AAV

Name 
AAV-h-PCCB-shRNA
Cat No 
shAAV-218315
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PCCB.

shAAV-218315
AAV-h-PCCB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PCCB (propionyl-CoA carboxylase beta subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Description (Vector)
PCCB is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
5096
Gene Name (Vector)
propionyl-CoA carboxylase beta subunit
Gene Symbol
PCCB
HGNC ID
HGNC:8654
ORF Size (aa)
1620
ORF Size (bp)
1620 bp
RefSeq ID
NM_000532
RefSeq Synonyms
NM_001178014, NM_000532, BC053661, BC013768,
Species
human
UniGene ID
Hs.63788

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.