human PAN3 shRNA silencing AAV

Name 
AAV-h-PAN3-shRNA
Cat No 
shAAV-218172
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PAN3.

shAAV-218172
AAV-h-PAN3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PAN3 (PAN3 poly(A) specific ribonuclease subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PAB1P-dependent poly(A)-nuclease; PAB1P-dependent poly(A)-specific ribonuclease; PAB-dependent poly(A)-specific ribonuclease subunit 3; PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PABP1-dependent poly A-specific ribonuclease subunit PAN3; PABP-dependent poly(A) nuclease 3; PAN deadenylation complex subunit 3; PAN2-PAN3 deadenylation complex subunit PAN3; PAN3 poly(A) specific ribonuclease subunit homolog; poly(A)-nuclease deadenylation complex subunit 3
Description (eCommerce)
PAB-dependent poly(A)-specific ribonuclease subunit PAN3 is a regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q58A45].
Gene ID
255967
Gene Name (eCommerce)
poly(A) specific ribonuclease subunit PAN3
Gene Name (Vector)
PAN3 poly(A) specific ribonuclease subunit
Gene Symbol
PAN3
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2064
ORF Size (bp)
2064 bp
Protein Name (eCommerce)
PAB-dependent poly(A)-specific ribonuclease subunit PAN3
RefSeq ID
NM_175854
RefSeq Synonyms
NM_175854, BC128180, BC128179,
Research Areas
Gene Expression,Kinase/Phosphatase,mRNA Processing,RNA Processing,Zinc-finger
Research Areas (Faceted)
cell_biology,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
PAB-dependent poly(A)-specific ribonuclease subunit PAN3 is a regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q58A45].
UniGene ID
Hs.645015
UniProt ID (eCommerce)
Q58A45

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.