human PABPN1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human PABPN1.
shAAV-218100
AAV-h-PABPN1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human PABPN1 (poly(A) binding protein nuclear 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Nuclear poly(A)-binding protein 1; OPMD; PAB2; PABII; PABP2; PABP-2; poly(A) binding protein 2; poly(A) binding protein II; Poly(A)-binding protein 2; Poly(A)-binding protein II; Polyadenylate-binding nuclear protein 1; polyadenylate-binding protein 2
Description (eCommerce)
Polyadenylate binding protein, nuclear 1 (PABPN1) is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. PABPN1 stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and also controls the poly(A) tail length. PABPN1 increases the affinity of poly(A) polymerase for RNA and is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86U42].
Description (Vector)
PABPN1 encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. PABPN1 contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
Gene ID
8106
Gene Name (eCommerce)
poly(A) binding protein nuclear 1
Gene Name (Vector)
poly(A) binding protein nuclear 1
Gene Symbol
PABPN1
HGNC ID
HGNC:8565
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
921
ORF Size (bp)
921 bp
Protein Name (eCommerce)
Polyadenylate-binding protein 2
RefSeq ID
NM_004643
RefSeq Synonyms
NM_004643, BC010939,
Research Areas
Gene Expression,Host-Virus Interactions,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,infectious_disease,cell_biology
Species
human
Target Sentence
Polyadenylate binding protein, nuclear 1 (PABPN1) is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. PABPN1 stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and also controls the poly(A) tail length. PABPN1 increases the affinity of poly(A) polymerase for RNA and is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86U42].
UniGene ID
Hs.707712
UniProt ID (eCommerce)
Q86U42
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.