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human PABPC4 shRNA silencing AAV

Name 
AAV-h-PABPC4-shRNA
Cat No 
shAAV-218097
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PABPC4.

shAAV-218097
AAV-h-PABPC4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PABPC4 (poly(A) binding protein cytoplasmic 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
activated-platelet protein 1; APP1; APP-1; inducible poly(A)-binding protein; iPABP; PABP4; PABP-4; poly(A) binding protein, cytoplasmic 4 (inducible form); poly(A)-binding protein 4; polyadenylate-binding protein 4
Description (eCommerce)
PABP4 (poly(A)-binding protein 4), also known as iPABP (inducible poly(A)-binding protein) was originally identified in a search for activation-induced genes in T-cells. It is one of five poly(A)-binding proteins encoded by independent genes whose products play roles in various aspects of mRNA processing. The PABP genes encode one nuclear and four cytoplasmic PABP proteins. PABP2/PABP (PABPN1) is the observed nuclear PABP. The cytoplasmic PABPs include PABP1 (PABPC1), PABP3 (PABPC3), PABP4/iPABP (PABPC4), and x-linked PABP5 (PABPC5). The cytoplasmic PABPs bear four RNA-recognition motifs (RRMs) that participate in binding poly (A). In T-cells, PABP4 is proposed to play a role in stabilizing labile mRNA.
Description (Vector)
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
8761
Gene Name (eCommerce)
poly(A) binding protein cytoplasmic 4
Gene Name (Vector)
poly(A) binding protein cytoplasmic 4
Gene Symbol
PABPC4
HGNC ID
HGNC:8557
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1896
ORF Size (bp)
1896 bp
Protein Name (eCommerce)
Polyadenylate-binding protein 4
RefSeq ID
NM_003819
RefSeq Synonyms
NM_003819, NM_001135654, NM_001135653, BC094755, BC071591, BC065540,
Research Areas
RNA Binding,RNA Processing,RNA Transport,Translational Control
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
PABP4 (poly(A)-binding protein 4), also known as iPABP (inducible poly(A)-binding protein) was originally identified in a search for activation-induced genes in T-cells. It is one of five poly(A)-binding proteins encoded by independent genes whose products play roles in various aspects of mRNA processing. The PABP genes encode one nuclear and four cytoplasmic PABP proteins. PABP2/PABP (PABPN1) is the observed nuclear PABP. The cytoplasmic PABPs include PABP1 (PABPC1), PABP3 (PABPC3), PABP4/iPABP (PABPC4), and x-linked PABP5 (PABPC5). The cytoplasmic PABPs bear four RNA-recognition motifs (RRMs) that participate in binding poly (A). In T-cells, PABP4 is proposed to play a role in stabilizing labile mRNA.
UniGene ID
Hs.169900
UniProt ID (eCommerce)
Q13310

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.