human P4HA2 shRNA silencing AAV

Name 
AAV-h-P4HA2-shRNA
Cat No 
shAAV-218077
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human P4HA2.

shAAV-218077
AAV-h-P4HA2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human P4HA2 (prolyl 4-hydroxylase, alpha polypeptide II). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4-PH alpha 2; 4-PH alpha-2; collagen prolyl 4-hydroxylase alpha(II); C-P4Halpha(II); MYP25; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; prolyl 4-hydroxylase subunit alpha-2; prolyl 4-hydroxylase, alpha polypeptide II
Description (eCommerce)
P4HA2 is a key enzyme in collagen synthesis that catalyzes the formation of 4-hydroxyproline which is essential to the folding of newly synthesized pro-collagen.
Description (Vector)
P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID
8974
Gene Name (eCommerce)
prolyl 4-hydroxylase subunit alpha 2
Gene Name (Vector)
prolyl 4-hydroxylase, alpha polypeptide II
Gene Symbol
P4HA2
HGNC ID
HGNC:8547
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1602
ORF Size (bp)
1602 bp
Protein Name (eCommerce)
Prolyl 4-hydroxylase subunit alpha-2
RefSeq ID
NM_004199
RefSeq Synonyms
NM_004199, NM_001142599, NM_001142598, NM_001017974, NM_001017973, BC035813, BC017062,
Research Areas
Immunology,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
cell_biology,immunology,metabolism,signal_transduction
Species
human
Target Sentence
P4HA2 is a key enzyme in collagen synthesis that catalyzes the formation of 4-hydroxyproline which is essential to the folding of newly synthesized pro-collagen.
UniGene ID
Hs.519568
UniProt ID (eCommerce)
O15460

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.