human ORC2L shRNA silencing AAV

Name 
AAV-h-ORC2L-shRNA
Cat No 
shAAV-217925
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ORC2.

shAAV-217925
AAV-h-ORC2L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ORC2 (origin recognition complex subunit 2). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ORC2L; origin recognition complex protein 2 homolog; origin recognition complex subunit 2; origin recognition complex, subunit 2 homolog
Description (eCommerce)
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC2 is also known as ORC2L and is a subunit of the human ORC complex. ORC2 forms a core complex with ORC3L, -4L, and -5L [taken from NCBI Entrez Gene (GeneID: 4999)].
Description (Vector)
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate.
Gene ID
4999
Gene Name (eCommerce)
origin recognition complex subunit 2
Gene Name (Vector)
origin recognition complex subunit 2
Gene Symbol
ORC2
HGNC ID
HGNC:8488
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1734
ORF Size (bp)
1734 bp
Protein Name (eCommerce)
Origin recognition complex subunit 2
RefSeq ID
NM_006190
RefSeq Synonyms
NM_006190, BC014834,
Research Areas
Cell Cycle,DNA Replication,Replication,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC2 is also known as ORC2L and is a subunit of the human ORC complex. ORC2 forms a core complex with ORC3L, -4L, and -5L [taken from NCBI Entrez Gene (GeneID: 4999)].
UniGene ID
Hs.444870
UniProt ID (eCommerce)
Q13416

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.