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human NUMA1 shRNA silencing AAV

Name 
AAV-h-NUMA1-shRNA
Cat No 
shAAV-217228
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NUMA1.

shAAV-217228
AAV-h-NUMA1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NUMA1 (nuclear mitotic apparatus protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; nuclear matrix protein-22; Nuclear mitotic apparatus protein; nuclear mitotic apparatus protein 1; NUMA; NuMA protein; SP-H antigen; structural nuclear protein
Description (eCommerce)
The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.
Gene ID
4926
Gene Name (eCommerce)
nuclear mitotic apparatus protein 1
Gene Name (Vector)
nuclear mitotic apparatus protein 1
Gene Symbol
NUMA1
HGNC ID
HGNC:8059
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3582
ORF Size (bp)
3582 bp
Protein Name (eCommerce)
Nuclear mitotic apparatus protein 1
RefSeq ID
NM_006185
RefSeq Synonyms
NM_006185, NM_001286561, BC013023,
Research Areas
Apoptosis,Cell Cycle,Cell Division,Cytoskeleton,Host-Virus Interactions
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,infectious_disease
Species
human
Target Sentence
The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.
UniGene ID
Hs.325978
UniProt ID (eCommerce)
Q14980

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.