human NTAN1 shRNA silencing AAV

Name 
AAV-h-NTAN1-shRNA
Cat No 
shAAV-217151
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NTAN1.

shAAV-217151
AAV-h-NTAN1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NTAN1 (N-terminal asparagine amidase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PNAA; PNAD
Description (Vector)
NTAN1 functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Gene ID
123803
Gene Name (Vector)
N-terminal asparagine amidase
Gene Symbol
NTAN1
HGNC ID
HGNC:29909
ORF Size (aa)
933
ORF Size (bp)
933 bp
RefSeq ID
NM_173474
RefSeq Synonyms
NM_173474, NM_001270767, NM_001270766, BC017336,
Species
human
UniGene ID
Hs.592045

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.