human NFS1 shRNA silencing AAV

Name 
AAV-h-NFS1-shRNA
Cat No 
shAAV-216659
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NFS1.

shAAV-216659
AAV-h-NFS1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NFS1 (NFS1, cysteine desulfurase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HUSSY-08; IscS; NIFS
Description (Vector)
Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.
Gene ID
9054
Gene Name (Vector)
NFS1, cysteine desulfurase
Gene Symbol
NFS1
HGNC ID
HGNC:15910
ORF Size (aa)
1374
ORF Size (bp)
1374 bp
RefSeq ID
NM_021100
RefSeq Synonyms
NM_021100, NM_001198989, BC065560,
Species
human
UniGene ID
Hs.194692

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.