human NFIL3 shRNA silencing AAV

Name 
AAV-h-NFIL3-shRNA
Cat No 
shAAV-216642
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NFIL3.

shAAV-216642
AAV-h-NFIL3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NFIL3 (nuclear factor, interleukin 3 regulated). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
E4 promoter-binding protein 4; E4BP4; IL3BP1; interleukin-3 promoter transcriptional activator; interleukin-3-binding protein 1; NFIL3A; NF-IL3A; nuclear factor interleukin-3-regulated protein; transcriptional activator NF-IL3A
Description (eCommerce)
E4 promoter-binding protein 4 (E4BP4) functions as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. E4BP4 activates transcription from the interleukin-3 promoter in T-cells and is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16649].
Description (Vector)
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 (PubMed 7565758)).(supplied by OMIM, Feb 2009)
Gene ID
4783
Gene Name (eCommerce)
nuclear factor, interleukin 3 regulated
Gene Name (Vector)
nuclear factor, interleukin 3 regulated
Gene Symbol
NFIL3
HGNC ID
HGNC:7787
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1389
ORF Size (bp)
1389 bp
Protein Name (eCommerce)
Nuclear factor interleukin-3-regulated protein
RefSeq ID
NM_005384
RefSeq Synonyms
NM_005384, NM_001290000, NM_001289999, BC008197,
Research Areas
Gene Expression,Immunology,Interleukin Signaling,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,signal_transduction,transcription_translation
Species
human
Target Sentence
E4 promoter-binding protein 4 (E4BP4) functions as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. E4BP4 activates transcription from the interleukin-3 promoter in T-cells and is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16649].
UniGene ID
Hs.79334
UniProt ID (eCommerce)
Q16649

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.