human NDUFA1 shRNA silencing AAV

Name 
AAV-h-NDUFA1-shRNA
Cat No 
shAAV-216470
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NDUFA1.

shAAV-216470
AAV-h-NDUFA1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NDUFA1 (NADH:ubiquinone oxidoreductase subunit A1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CI-MWFE; MWFE; ZNF183
Description (Vector)
The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the 'hydrophobic protein' (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Gene ID
4694
Gene Name (Vector)
NADH:ubiquinone oxidoreductase subunit A1
Gene Symbol
NDUFA1
HGNC ID
HGNC:7683
ORF Size (aa)
213
RefSeq ID
NM_004541
RefSeq Synonyms
NM_004541, BC000266,
Species
human
UniGene ID
Hs.534168

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.