human NAE1 shRNA silencing AAV

Name
AAV-h-NAE1-shRNA

Cat No
shAAV-216285

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NAE1.

shAAV-216285

AAV-h-NAE1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NAE1 (NEDD8 activating enzyme E1 subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

A-116A10.1; APPBP1; HPP1; ula-1

Description (Vector)

NAE1 binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

Gene ID

8883

Gene Name (Vector)

NEDD8 activating enzyme E1 subunit 1

Gene Symbol

NAE1

HGNC ID

HGNC:621

ORF Size (aa)

1605

ORF Size (bp)

1605 bp

RefSeq ID

NM_003905

RefSeq Synonyms

NM_003905, NM_001286500, NM_001018160, NM_001018159, BC013301, BC000480,

Species

human

UniGene ID

Hs.460978

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.