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human MYOF shRNA silencing AAV

Name 
AAV-h-MYOF-shRNA
Cat No 
shAAV-216229
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MYOF.

shAAV-216229
AAV-h-MYOF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MYOF (myoferlin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
FER1L3; fer-1-like 3, myoferlin; fer-1-like family member 3; fer-1-like protein 3; myoferlin
Description (eCommerce)
Myoferlin is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Myoferlin is also Involved in endocytic recycling and implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NZM1].
Description (Vector)
Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined.
Gene ID
26509
Gene Name (eCommerce)
myoferlin
Gene Name (Vector)
myoferlin
Gene Symbol
MYOF
HGNC ID
HGNC:3656
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4734
ORF Size (bp)
4734 bp
Protein Name (eCommerce)
Myoferlin
RefSeq ID
NM_013451
RefSeq Synonyms
NM_133337, NM_013451, BC052617,
Research Areas
Cardiovascular,DNA Damage/Repair,Signal Transduction,VEGF Signaling
Research Areas (Faceted)
cardiovascular,cell_biology,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
Myoferlin is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Myoferlin is also Involved in endocytic recycling and implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NZM1].
UniGene ID
Hs.602086
UniProt ID (eCommerce)
Q9NZM1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.