human MRPL35 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MRPL35.
shAAV-215810
AAV-h-MRPL35-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MRPL35 (mitochondrial ribosomal protein L35). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
L35mt; MRP-L35
Description (Vector)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp.
Gene ID
51318
Gene Name (Vector)
mitochondrial ribosomal protein L35
Gene Symbol
MRPL35
HGNC ID
HGNC:14489
ORF Size (aa)
513
RefSeq ID
NM_016622
RefSeq Synonyms
NM_145644, NM_016622, BC020651,
Species
human
UniGene ID
Hs.433439
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.