human MRPL15 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MRPL15.
shAAV-215784
AAV-h-MRPL15-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MRPL15 (mitochondrial ribosomal protein L15). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
HSPC145; L15mt; MRP-L15; MRP-L7; RPML7
Description (Vector)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.
Gene ID
29088
Gene Name (Vector)
mitochondrial ribosomal protein L15
Gene Symbol
MRPL15
HGNC ID
HGNC:14054
ORF Size (aa)
891
ORF Size (bp)
891 bp
RefSeq ID
NM_014175
RefSeq Synonyms
NM_014175, BC000891,
Species
human
UniGene ID
Hs.18349
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.