human MRPL11 shRNA silencing AAV

Name
AAV-h-MRPL11-shRNA

Cat No
shAAV-215778

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MRPL11.

shAAV-215778

AAV-h-MRPL11-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MRPL11 (mitochondrial ribosomal protein L11). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CGI-113; L11MT; MRP-L11

Description (Vector)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 5q and 12q.

Gene ID

65003

Gene Name (Vector)

mitochondrial ribosomal protein L11

Gene Symbol

MRPL11

HGNC ID

HGNC:14042

ORF Size (aa)

501

RefSeq ID

NM_170738

RefSeq Synonyms

NM_170739, NM_170738, NM_016050, BC108277, BC005002,

Species

human

UniGene ID

Hs.418450

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.