human MLH1 shRNA silencing AAV

Name
AAV-h-MLH1-shRNA

Cat No
shAAV-215519

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MLH1.

shAAV-215519

AAV-h-MLH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MLH1 (mutL homolog 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

COCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; HNPCC; HNPCC2; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1

Description (eCommerce)

MLH1 is an integral component of the DNA mismatch repair (MMR) system that functions to recognize aberrant structures and signals for the repair of single base mismatches or loops in replicated DNA. MLH1 can heterodimerize with DNA mismatch repair protein MLH3 and with mismatch repair endonuclease PMS2. [taken from NCBI Entrez Gene (Gene ID: 4292)].

Description (Vector)

MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.

Gene ID

4292

Gene Name (eCommerce)

mutL homolog 1

Gene Name (Vector)

mutL homolog 1

Gene Symbol

MLH1

HGNC ID

HGNC:7127

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2271

ORF Size (bp)

2271 bp

Protein Name (eCommerce)

DNA mismatch repair protein Mlh1

RefSeq ID

NM_000249

RefSeq Synonyms

NM_001258274, NM_001258273, NM_001258271, NM_001167619, NM_001167618, NM_001167617, NM_000249, BC006850,

Research Areas

Apoptosis,Cancer,Cell Cycle,Colorectal Cancer,DNA Damage/Repair,Fanconi anemia,Hypoxia,Immunology,Spermatogenesis,Tumor Suppressors/Oncoproteins

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,developmental_biology,genetics,immunology,transcription_translation

Species

human

Target Sentence

MLH1 is an integral component of the DNA mismatch repair (MMR) system that functions to recognize aberrant structures and signals for the repair of single base mismatches or loops in replicated DNA. MLH1 can heterodimerize with DNA mismatch repair protein MLH3 and with mismatch repair endonuclease PMS2. [taken from NCBI Entrez Gene (Gene ID: 4292)].

UniGene ID

Hs.195364

UniProt ID (eCommerce)

P40692

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.