human MITF shRNA silencing AAV

Name
AAV-h-MITF-shRNA

Cat No
shAAV-215490

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MITF.

shAAV-215490

AAV-h-MITF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MITF (melanogenesis associated transcription factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

bHLHe32; CMM8; MI; WS2; WS2A

Description (Vector)

MITF encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene ID

4286

Gene Name (Vector)

melanogenesis associated transcription factor

Gene Symbol

MITF

HGNC ID

HGNC:7105

ORF Size (aa)

1260

ORF Size (bp)

1260 bp

RefSeq ID

NM_000248

RefSeq Synonyms

NM_198178, NM_198177, NM_198159, NM_198158, NM_006722, NM_001184968, NM_001184967, NM_000248, BC065243, BC026961, BC012503,

Species

human

UniGene ID

Hs.618266

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.