human MEST shRNA silencing AAV

Name
AAV-h-MEST-shRNA

Cat No
shAAV-215317

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MEST.

shAAV-215317

AAV-h-MEST-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MEST (mesoderm specific transcript). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PEG1

Description (Vector)

MEST encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.

Gene ID

4232

Gene Name (Vector)

mesoderm specific transcript

Gene Symbol

MEST

HGNC ID

HGNC:7028

ORF Size (aa)

879

ORF Size (bp)

879 bp

RefSeq ID

NM_002402

RefSeq Synonyms

NM_177525, NM_177524, NM_002402, NM_001253902, NM_001253901, NM_001253900, BC090049, BC018695, BC014564, BC011908, BC002413,

Species

human

UniGene ID

Hs.270978

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.