Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human MED27 shRNA silencing AAV

Name 
AAV-h-MED27-shRNA
Cat No 
shAAV-215260
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MED27.

shAAV-215260
AAV-h-MED27-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MED27 (mediator complex subunit 27). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CRAP34; CRSP34; CRSP8; MED3; TRAP37
Description (Vector)
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Gene ID
9442
Gene Name (Vector)
mediator complex subunit 27
Gene Symbol
MED27
HGNC ID
HGNC:2377
ORF Size (aa)
936
RefSeq ID
NM_004269
RefSeq Synonyms
NM_004269, NM_001253882, NM_001253881, BC064608,
Species
human
UniGene ID
Hs.374262

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.