human MED14 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MED14.
shAAV-215239
AAV-h-MED14-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MED14 (mediator complex subunit 14). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; hRGR1; human homolog of yeast RGR1; Mediator complex subunit 14; mediator of RNA polymerase II transcription subunit 14; RGR1; RGR1 homolog; thyroid hormone receptor-associated protein complex component TRAP170; Transcriptional coactivator CRSP150; TRAP170; vitamin D receptor-interacting protein complex component DRIP150; vitamin D3 receptor-interacting protein complex 150 kDa component
Description (eCommerce)
CRSP2 is a subunit of the CRSP (cofactor required for SP1 activation) complex required for efficient transcriptional activation by the SP1 transcription factor. It has also been identified as a component of the mediator complex that bridges nuclear hormone receptors to RNA polymerase II.
Description (Vector)
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
Gene ID
9282
Gene Name (eCommerce)
mediator complex subunit 14
Gene Name (Vector)
mediator complex subunit 14
Gene Symbol
MED14
HGNC ID
HGNC:2370
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4365
ORF Size (bp)
4365 bp
Protein Name (eCommerce)
Mediator of RNA polymerase II transcription subunit 14
RefSeq ID
NM_004229
RefSeq Synonyms
NM_004229, BC132674, BC132672, BC098377,
Research Areas
Gene Expression,Metabolism/Metabolic Process,Nuclear Receptor Coactivators,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,metabolism,signal_transduction,transcription_translation
Species
human
Target Sentence
CRSP2 is a subunit of the CRSP (cofactor required for SP1 activation) complex required for efficient transcriptional activation by the SP1 transcription factor. It has also been identified as a component of the mediator complex that bridges nuclear hormone receptors to RNA polymerase II.
UniGene ID
Hs.407604
UniProt ID (eCommerce)
O60244
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.