human MECP2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MECP2.
shAAV-215229
AAV-h-MECP2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MECP2 (methyl-CpG binding protein 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT
Description (Vector)
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Gene ID
4204
Gene Name (Vector)
methyl-CpG binding protein 2
Gene Symbol
MECP2
HGNC ID
HGNC:6990
ORF Size (aa)
1461
ORF Size (bp)
1461 bp
RefSeq ID
NM_004992
RefSeq Synonyms
NM_004992, NM_001316337, NM_001110792, BC011612,
Species
human
UniGene ID
Hs.200716
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.