human MAPKAP1 shRNA silencing AAV

Name 
AAV-h-MAPKAP1-shRNA
Cat No 
shAAV-214995
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MAPKAP1.

shAAV-214995
AAV-h-MAPKAP1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MAPKAP1 (mitogen-activated protein kinase associated protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
JC310; MEKK2-interacting protein 1; MIP1; mitogen-activated protein kinase 2-associated protein 1; mSIN1; ras inhibitor MGC2745; SAPK-interacting protein 1; SIN1; SIN1b; SIN1g; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1
Description (eCommerce)
Stress-activated map kinase-interacting protein 1 (Sin1) is highly similar to the yeast stress- activated protein kinase SIN1. Sin1 is an essential mTORC2 subunit that is required to act as the PDK2 that phosphorylates Akt/PKB at Ser 473. Sin1 has been implicated as a scaffolding protein. Sin1 is required for rictor binding in the mTORC2 complex. Sin1 has also been shown to be important to the SAPK (stress-activated kinase) signaling pathway and in the nucleus may act as a scaffold between ATF-2 and p38 to facilitate p38-induced phosphorylation of ATF-2. Sin1 is also found to form a complex with c-Jun N-terminal kinase (JNK) and may similarly function as a scaffolding protein in JNK signaling.
Description (Vector)
MAPKAP1 encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.
Gene ID
79109
Gene Name (eCommerce)
mitogen-activated protein kinase associated protein 1
Gene Name (Vector)
mitogen-activated protein kinase associated protein 1
Gene Symbol
MAPKAP1
HGNC ID
HGNC:18752
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1461
ORF Size (bp)
1461 bp
Protein Name (eCommerce)
Target of rapamycin complex 2 subunit MAPKAP1
RefSeq ID
NM_024117
RefSeq Synonyms
NM_024117, NM_001006621, NM_001006620, NM_001006619, NM_001006618, NM_001006617, BC003044, BC002326,
Research Areas
B-cell Receptor Signaling,Cancer,Cytoskeleton,EGFR Signaling,EGFR Signaling in Cancer,Fc Receptor Signaling,FGF Signaling,IFN Signaling,Immunology,Innate Immunity,mTOR Signaling,Neurobiology,NGF Signaling,PDGF Signaling,PI3K/Akt Signaling,PI3K-AKT Signaling in Cancer,SCF-KIT Signaling,Signal Transduction
Research Areas (Faceted)
cancer,cell_biology,immunology,neurobiology,signal_transduction,stem_cell_biology
Species
human
Target Sentence
Stress-activated map kinase-interacting protein 1 (Sin1) is highly similar to the yeast stress- activated protein kinase SIN1. Sin1 is an essential mTORC2 subunit that is required to act as the PDK2 that phosphorylates Akt/PKB at Ser 473. Sin1 has been implicated as a scaffolding protein. Sin1 is required for rictor binding in the mTORC2 complex. Sin1 has also been shown to be important to the SAPK (stress-activated kinase) signaling pathway and in the nucleus may act as a scaffold between ATF-2 and p38 to facilitate p38-induced phosphorylation of ATF-2. Sin1 is also found to form a complex with c-Jun N-terminal kinase (JNK) and may similarly function as a scaffolding protein in JNK signaling.
UniGene ID
Hs.495138
UniProt ID (eCommerce)
Q9BPZ7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.