human MAP3K5 shRNA silencing AAV

Name
AAV-h-MAP3K5-shRNA

Cat No
shAAV-214936

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MAP3K5.

shAAV-214936

AAV-h-MAP3K5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MAP3K5 (mitogen-activated protein kinase kinase kinase 5). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ASK1; MAPKKK5; MEKK5

Description (Vector)

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Gene ID

4217

Gene Name (Vector)

mitogen-activated protein kinase kinase kinase 5

Gene Symbol

MAP3K5

HGNC ID

HGNC:6857

ORF Size (aa)

4125

ORF Size (bp)

4125 bp

RefSeq ID

NM_005923

RefSeq Synonyms

NM_005923, BC088829, BC054503,

Species

human

UniGene ID

Hs.186486

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.